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KMID : 1094720060110050455
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 5 p.455 ~ p.461
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Fabrication of disposable protein chip for simultaneous sample detection
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Lee Chang-Soo
Lee Sang-Ho
Kim Yun-Gon
Oh Min-Kyu
Hwang Taek-Sung
Rhee Young-Woo
Song Hwan-Moon
Kim Bo-Yeol
Kim Yong-Kweon
Kim Byung-Gee
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Abstract
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In this study, we have described a method for the fabrication of a protein chip on silicon substrate using hydrophobic thin film and microfluidic channels, for the simultaneous detection of multiple targets in samples. The use of hydrophobic thin film provides for a physical, chemical, and biological barrier for protein patterning. The microfluidic channels create four protein patterned strips on the silicon surfaces with a high signal-to-noise ratio. The feasibility of the protein chips was determined in order to discriminate between each protein interaction in a mixture sample that included biotin, ovalbumin, hepatitis B antigen. In the fabrication of the multiplexed assay system, the utilization of the hydrophobic thin film and the microfluidic networks constitutes a more convenient method for the development of biosensors or biochips. This technique may be applicable to the simultaneous evaluation of multiple protein-protein interactions.
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KEYWORD
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protein chip, protein patterning, microchannel, simultaneous detection
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